Resources

Request Mice from Sharpe Lab

To request research mice from Sharpe lab please send an email to sharpe_lab@hms.harvard.edu with the following information:

  • Name and contact information for the principal investigator
  • Requested strain
  • Preferred background
  • Description of proposed research to initiate a Material Transfer Agreement (MTA)

Recipients are responsible for all shipping costs plus a $200 packing and materials fee.

A number of our strains are available from the Jackson Labs and the MMRRC:

Jackson Strain Stock #
NOD.B6-Cd274tm1Shr/J 18307  
BALBc.Icos 017605
B6.CTLA4/CD28  017607
129S4/SvJae-Cd274tm2Shr Pdcd1lg2tm4Shr/Mmjax 012674
129S4/SvJae-Pdcd1lg2tm1Shr/Mmjax  012676
B6.129S4-Cd80tm1Shr Cd86tm2Shr/J 003610
NOD.B6-Cd274tm1Shr/J 018307
B6.129S4-Cd28tm1Shr Ctla4tm1Shr/J 017607
B6.Cg-Pdcd1tm1.1Shr/J 021157
MMRrC Strain Stock #
129S4-Ctla4tm1Shr Cd28tm1Shr/Mmnc MMRRC:030492-UNC
129S4/SvJae-Cd274tm2 Pdcd1lg2tm4Shr/Mmjax  MMRRC:032239-JAX
29S4/SvJae-Cd274tm2Shr/Mmjax MMRRC:032234-JAX
129S4/SvJae-Cd274tm2Shr/Mmjax MMRRC:032234-JAX
129S4/SvJae-Cd28tm1Shr/Mmucd MMRRC:010628-UCD
129S4/SvJae-Cd80tm1Shr Cd86tm1Shr Ctla4tm1Shr/Mmmh MMRRC:011791-MU
129S4/SvJae-Cd80tm1Shr Cd86tm2Shr/Mmnc MMRRC:010625-UNC
129S4/SvJae-Cd80tm1Shr/Mmnc MMRRC:010623-UNC
129S4/SvJae-Cd86tm1Shr/Mmnc MMRRC:010624-UNC
129S4/SvJae-Icostm1Shr/Mmucd MMRRC:010627-UCD
129S4/SvJae-Pdcd1lg2tm2Shr/Mmjax MMRRC:032235-JAX
129S4/SvJae-Tnfsf4tm1Shr/Mmnc MMRRC:010626-UNC
B6.129S4-Tnfsf4tm1Shr/Mmmh MMRRC:011140-MU
B6;129S4-Tmem123tm1Shr/Mmnc MMRRC:030494-UNC
C.129-Cd40tm1Kik Cd86tm2Shr Cd80tm1Shr/Mmnc MMRRC:011489-UNC
C.129S4-Cd80tm1Shr Cd86tm1Shr/Mmmh MMRRC:011139-MU
C.129S4-Cd80tm1Shr/Mmmh MMRRC:011137-MU
C.129S4-Cd86tm1Shr/Mmmh MMRRC:011138-MU
C.129S4-Ctla4tm1Shr Cd28tm1Shr/Mmnc MMRRC:030493-UNC
SJL.129S4-Cd80tm1Shr Cd86tm1Shr/Mmmh MMRRC:011790-MU
SJL.129S4-Cd80tm1Shr/Mmmh MMRRC:011788-MU
SJL.129S4-Cd86tm1Shr/Mmmh MMRRC:011789-MU                                                                  

                                                                

Department Resources

The HMS Department of Microbiology and Immunobiology has a microscopy suite with a variety of microscopes including confocal and fluorescent microscopes. Additional shared equipment includes several ultracentrifuges, Sorvall centrifuges, beta scintillation and gamma counters, spectrophotometers, real time RT-PCR machines, a nCounter for gene analysis, and a dark room with automatic film processor.

DF/HCC Core Facilities

The DF/HCC Core Facilities are central or “shared” laboratories that provide members critical services to advance their research. Each of the cores is capable of performing a specific set of experimental functions that enable DF/HCC investigators to perform experiments faster and more accurately. The core facilities are a cornerstone of DF/HCC, and they promote cross-institutional collaboration and the effective translation of discoveries into novel approaches to cancer research.

DNA Resource Core

The Dana-Farber/Harvard Cancer Center DNA Resource Core provides DNA sequencing services for large- and small-scale projects and a plasmid repository and distribution service with high quality and in a timely fashion. Typically, there is a 24-hour turn-around from sample pick-up to data availability. 

Flow Cytometry Core

The HMS Department of Microbiology and Immunobiology has a flow cytometry facility with cell analysis and sorting capabilities. The facility had an LSR-II analyzer with three lasers and a high throughput sampler for multiparameter flow analysis, FACS-Calibur for routine four-color analysis, a FACSAria for high speed cell sorting equipped with four laser and detectors for 14-color discrimination and 4-way sorting, and a 4 laser MoFlo Astrios cell sorter with 18 parameter 6 way sorting.

Longwood Medical Area CyTOF Core

The CyTOF Core is a multi-institutional core facility. It is located at the Dana-Farber Cancer Institute and staffed with a dedicated CyTOF operator familiar with instrument operation, CyTOF staining, and data analysis. A dedicated users group is optimizing protocols for preparation of metal-tagged antibodies and staining.

Rodent Histopathology Core

The Dana-Farber/Harvard Cancer Center Rodent Histopathology Core focuses on non-human pathology, primarily rodent, and performs conventional histopathology of paraffin-embedded, hematoxylin, and eosin stained slides as well as conventional special stains such as PAS, trichrome, and luxol fast blue/cresyl violet.

Transgenic Mouse Core

The Transgenic Mouse Core, overseen by Dr. Arlene Sharpe, provides gene targeting and microinjection services. This includes microinjection of small or BAC-sized DNA constructs for transgenic mouse generation, microinjection of lentiviral vectors for lentigenics, and CRISPR/Cas9 mediated mouse engineering.  The core will also generate targeted ES cell lines using vectors provided by investigators and inject these targeted ES cells, or targeted ES cells purchased by investigators.

More information can be found on the HMS Division of Immunology website under Resources.