A clinical method for the determination of serum gamma-glutamyl transpeptidase
Publication information:
Tamaoki, Minato, Takei, Fujisawa. A clinical method for the determination of serum gamma-glutamyl transpeptidase. Clin Chim Acta. 1975;65(1):21–7.
Abstract
A simple, highly sensitive and reproducible method for the assay of gamma-glutamyl transpeptidase (EC 2.3.2-) activity is introduced, using gamma-glutamyl-p-nitroanilide as a substrate and glycylglycine as an acceptor in 50 g/l of polyoxyethylene nonylphenol. Serum transpeptidase activity was assayed in 1080 healthy adults, the normal mean value being 14.8 mU/ml. The diagnostic evaluation of the enzyme in various hepatobiliary diseases is also discussed.