# Resources ![Pipetting](/sites/g/files/omnuum4061/files/sharpe/files/rsz_1evergrande-symposium-107_copy_2.jpg) ![PCR tubes](/sites/g/files/omnuum4061/files/sharpe/files/rsz_evergrande-symposium-123_copy_2.jpg) ![Samples being stored](/sites/g/files/omnuum4061/files/sharpe/files/rsz_1evergrande-symposium-128_copy_2.jpg) ### Request Mice from Sharpe Lab expand\_more To request research mice from Sharpe lab please send an email to [Cecilia\_Washburn@hms.harvard.edu](mailto:Cecilia_Washburn@hms.harvard.edu) with the following information: - Name and contact information for the principal investigator - Requested strain - Preferred background - Description of proposed research to initiate a Material Transfer Agreement (MTA) Recipients are responsible for all shipping costs plus a $200 packing and materials fee. **A number of our strains are available from the Jackson Labs and the MMRRC:** Sort**Jackson Strain** **Stock #** [B6.Cg-Cd274tm3.1Shr/J](https://www.jax.org/strain/036255) 036255 [NOD.B6-Cd274tm1Shr/J](https://www.jax.org/strain/018307) 18307 [BALBc.Icos](https://www.jax.org/strain/017605) 017605 [B6.CTLA4/CD28](https://www.jax.org/strain/017607) 017607 [129S4/SvJae-*Cd274tm2Shr Pdcd1lg2tm4Shr*/Mmjax](https://www.jax.org/strain/012674) 012674 [129S4/SvJae-*Pdcd1lg2tm1Shr*/Mmjax](https://www.jax.org/strain/012676) 012676 [B6.129S4-*Cd80tm1Shr Cd86tm2Shr*/J](https://www.jax.org/strain/003610) 003610 [NOD.B6-*Cd274tm1Shr*/J](https://www.jax.org/strain/018307) 018307 [B6.129S4-*Cd28tm1Shr Ctla4tm1Shr*/J](https://www.jax.org/strain/017607) 017607 [B6.Cg-*Pdcd1tm1.1Shr*/J](https://www.jax.org/strain/021157) 021157 [C57BL/6-*Cd274tm1Shr*/J](https://www.jax.org/strain/040705) 040705 [B6.Cg-*Pdcd1lg2tm1(Hbegf)Xzng*/ShrJ](https://www.jax.org/strain/040710) 040710 [B6.Cg-*Ptprca Pepcb Gt(ROSA)26Sortm1.1(CAG-cas9\*,-EGFP)Fezh* Tg(TcrLCMV)327Sdz/ShrJ](https://www.jax.org/strain/040736) 040736 [B6.Cg-*Ptprca Pepcb Gt(ROSA)26Sortm1.1(CAG-cas9\*,-EGFP)Fezh* Tg(TcraTcrb)425Cbn/ShrJ](https://www.jax.org/strain/040737) 040737 Sort**MMRrC Strain** **Stock #** [129S4-*Ctla4tm1Shr Cd28tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=30492) MMRRC:030492-UNC [129S4/SvJae-*Cd274tm2 Pdcd1lg2tm4Shr*/Mmjax](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=32239) MMRRC:032239-JAX [29S4/SvJae-*Cd274tm2Shr*/Mmjax](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=32234) MMRRC:032234-JAX [129S4/SvJae-*Cd274tm2Shr*/Mmjax](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=32234) MMRRC:032234-JAX [129S4/SvJae-*Cd28tm1Shr*/Mmucd](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10628) MMRRC:010628-UCD [129S4/SvJae-*Cd80tm1Shr Cd86tm1Shr Ctla4tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11791) MMRRC:011791-MU [129S4/SvJae-*Cd80tm1Shr Cd86tm2Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10625) MMRRC:010625-UNC [129S4/SvJae-*Cd80tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10623) MMRRC:010623-UNC [129S4/SvJae-*Cd86tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10624) MMRRC:010624-UNC [129S4/SvJae-*Icostm1Shr*/Mmucd](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10627) MMRRC:010627-UCD [129S4/SvJae-*Pdcd1lg2tm2Shr*/Mmjax](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=32235) MMRRC:032235-JAX [129S4/SvJae-*Tnfsf4tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=10626) MMRRC:010626-UNC [B6.129S4-*Tnfsf4tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11140) MMRRC:011140-MU [B6;129S4-*Tmem123tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=30494) MMRRC:030494-UNC [C.129-*Cd40tm1Kik Cd86tm2Shr Cd80tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11489) MMRRC:011489-UNC [C.129S4-*Cd80tm1Shr Cd86tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11139) MMRRC:011139-MU [C.129S4-*Cd80tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11137) MMRRC:011137-MU [C.129S4-*Cd86tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11138) MMRRC:011138-MU [C.129S4-*Ctla4tm1Shr Cd28tm1Shr*/Mmnc](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=30493) MMRRC:030493-UNC [SJL.129S4-*Cd80tm1Shr Cd86tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11790) MMRRC:011790-MU [SJL.129S4-*Cd80tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11788) MMRRC:011788-MU [SJL.129S4-*Cd86tm1Shr*/Mmmh](https://www.mmrrc.org/catalog/getSDS.php?mmrrc_id=11789) MMRRC:011789-MU ### Repositories expand\_more Many of our published materials are available through Addgene: [https://www.addgene.org/Arlene\_Sharpe/](https://www.addgene.org/Arlene_Sharpe/) ### Department Resources expand\_more The HMS Department of Immunology has a microscopy suite with a variety of microscopes including confocal and fluorescent microscopes. Additional shared equipment includes several ultracentrifuges, Sorvall centrifuges, beta scintillation and gamma counters, spectrophotometers, real time RT-PCR machines, a nCounter for gene analysis, and a dark room with automatic film processor. ### Flow Cytometry Core expand\_more The HMS Department of Immunology has a [flow cytometry facility](https://immunology.hms.harvard.edu/resources/flow-cytometry) with cell analysis and sorting capabilities. The facility had an LSR-II analyzer with three lasers and a high throughput sampler for multiparameter flow analysis, FACS-Calibur for routine four-color analysis, a FACSAria for high speed cell sorting equipped with four laser and detectors for 14-color discrimination and 4-way sorting, and a 4 laser MoFlo Astrios cell sorter with 18 parameter 6 way sorting. ### Mouse Engineering Core expand\_more The [Mouse Engineering Core](https://www.dfhcc.harvard.edu/research/core-facilities/mouse-engineering-core/), overseen by Dr. Arlene Sharpe, provides gene targeting and microinjection services. This includes microinjection of small or BAC-sized DNA constructs for transgenic mouse generation, microinjection of lentiviral vectors for lentigenics, and CRISPR/Cas9 mediated mouse engineering. The core will also generate targeted ES cell lines using vectors provided by investigators and inject these targeted ES cells, or targeted ES cells purchased by investigators. ### Rodent Histopathology Core expand\_more The Dana-Farber/Harvard Cancer Center [Rodent Histopathology Core](http://www.dfhcc.harvard.edu/research/core-facilities/rodent-histopathology/) focuses on non-human pathology, primarily rodent, and performs conventional histopathology of paraffin-embedded, hematoxylin, and eosin stained slides as well as conventional special stains such as PAS, trichrome, and luxol fast blue/cresyl violet. ### Longwood Medical Area CyTOF Core expand\_more The [CyTOF Core](http://hits.harvard.edu/lma-cytof-core-facility-information/) is a multi-institutional core facility. It is located at the Dana-Farber Cancer Institute and staffed with a dedicated CyTOF operator familiar with instrument operation, CyTOF staining, and data analysis. A dedicated users group is optimizing protocols for preparation of metal-tagged antibodies and staining. ### DF/HCC Core Facilities expand\_more The [DF/HCC Core Facilities](http://www.dfhcc.harvard.edu/research/core-facilities/) are central or “shared” laboratories that provide members critical services to advance their research. Each of the cores is capable of performing a specific set of experimental functions that enable DF/HCC investigators to perform experiments faster and more accurately. The core facilities are a cornerstone of DF/HCC, and they promote cross-institutional collaboration and the effective translation of discoveries into novel approaches to cancer research.