%0 Journal Article %J J Clin Invest %D 2010 %T PD-L1 has distinct functions in hematopoietic and nonhematopoietic cells in regulating T cell responses during chronic infection in mice. %A Mueller, Scott N %A Vanguri, Vijay K %A Ha, Sang-Jun %A West, Erin E %A Keir, Mary E %A Glickman, Jonathan N %A Sharpe, Arlene H %A Ahmed, Rafi %K Animals %K Bone Marrow %K Chronic Disease %K Female %K Ligands %K Lymphocyte Count %K Lymphocytic Choriomeningitis %K Mice %K Mice, Inbred C57BL %K Mice, Knockout %K T-Lymphocytes %K Virus Diseases %X The inhibitory receptor programmed death 1 (PD-1) is upregulated on antigen-specific CD8+ T cells during persistent viral infections. Interaction with PD-1 ligand 1 (PD-L1) contributes to functional exhaustion of responding T cells and may limit immunopathology during infection. PD-L1 is expressed on both hematopoietic and nonhematopoietic cells in tissues. However, the exact roles of PD-L1 on hematopoietic versus nonhematopoietic cells in modulating immune responses are unclear. Here we used bone marrow chimeric mice to examine the effects of PD-L1 deficiency in hematopoietic or nonhematopoietic cells during lymphocytic choriomeningitis virus clone 13 (LCMV CL-13) infection. We found that PD-L1 expression on hematopoietic cells inhibited CD8+ T cell numbers and function after LCMV CL-13 infection. In contrast, PD-L1 expression on nonhematopoietic cells limited viral clearance and immunopathology in infected tissues. Together, these data demonstrate that there are distinct roles for PD-L1 on hematopoietic and nonhematopoietic cells in regulating CD8+ T cell responses and viral clearance during chronic viral infection. %B J Clin Invest %V 120 %P 2508-15 %8 2010 Jul %G eng %N 7 %1 http://www.ncbi.nlm.nih.gov/pubmed/20551512?dopt=Abstract %R 10.1172/JCI40040