@article {542776,
	title = {B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4.},
	journal = {J Clin Invest},
	volume = {107},
	number = {7},
	year = {2001},
	month = {2001 Apr},
	pages = {881-7},
	abstract = {To examine whether B7 costimulation can be mediated by a molecule on T cells that is neither CD28 nor CTLA4, we generated mice lacking both of these receptors. CD28/CTLA4(-/-) mice resemble CD28(-/-) mice in having decreased expression of T-cell activation markers in vivo and decreased T-cell proliferation in vitro, as compared with wild-type mice. Using multiple approaches, we find B7-dependent costimulation in CD28/CTLA4(-/-) mice. The proliferation of CD28/CTLA4(-/-) T cells is inhibited by CTLA4-Ig and by the use of antigen-presenting cells lacking both B7-1 and B7-2. CD28/CTLA4(-/-) T-cell proliferation is increased by exposure to Chinese hamster ovary cells transfected with B7-1 or B7-2. Finally, administration of CTLA4-Ig to CD28/CTLA4(-/-) cardiac allograft recipients significantly prolongs graft survival. These data support the existence of an additional receptor for B7 molecules that is neither CD28 nor CTLA4.},
	keywords = {Animals, Antigens, CD, Antigens, CD28, Antigens, CD80, Antigens, CD86, Antigens, Differentiation, Cell Division, CHO Cells, Cricetinae, CTLA-4 Antigen, Graft Rejection, Graft Survival, Heart Transplantation, Immunoconjugates, Immunophenotyping, Interferon-gamma, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, T-Lymphocytes, Th1 Cells, Transplantation, Homologous},
	issn = {0021-9738},
	doi = {10.1172/JCI11710},
	author = {Mandelbrot, D A and Oosterwegel, M A and Shimizu, K and Yamada, A and Freeman, G. J. and Mitchell, R N and Sayegh, M H and Sharpe, A. H.}
}
