@article {542086,
	title = {GEF-H1 controls microtubule-dependent sensing of nucleic acids for antiviral host defenses.},
	journal = {Nat Immunol},
	volume = {15},
	number = {1},
	year = {2014},
	month = {2014 Jan},
	pages = {63-71},
	abstract = {Detailed understanding of the signaling intermediates that confer the sensing of intracellular viral nucleic acids for induction of type I interferons is critical for strategies to curtail viral mechanisms that impede innate immune defenses. Here we show that the activation of the microtubule-associated guanine nucleotide exchange factor GEF-H1, encoded by Arhgef2, is essential for sensing of foreign RNA by RIG-I-like receptors. Activation of GEF-H1 controls RIG-I-dependent and Mda5-dependent phosphorylation of IRF3 and induction of IFN-β expression in macrophages. Generation of Arhgef2(-/-) mice revealed a pronounced signaling defect that prevented antiviral host responses to encephalomyocarditis virus and influenza A virus. Microtubule networks sequester GEF-H1 that upon activation is released to enable antiviral signaling by intracellular nucleic acid detection pathways.},
	keywords = {Animals, Cercopithecus aethiops, COS Cells, DEAD-box RNA Helicases, Gene Expression, Green Fluorescent Proteins, HEK293 Cells, Humans, Immunity, Innate, Immunoblotting, Influenza A virus, Interferon Regulatory Factor-3, Interferon-beta, Macrophages, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Confocal, Microtubules, Phosphorylation, Reverse Transcriptase Polymerase Chain Reaction, Rho Guanine Nucleotide Exchange Factors, RNA, Viral, Signal Transduction},
	issn = {1529-2916},
	doi = {10.1038/ni.2766},
	author = {Chiang, Hao-Sen and Zhao, Yun and Song, Joo-Hye and Liu, Song and Wang, Ninghai and Terhorst, Cox and Sharpe, Arlene H and Basavappa, Megha and Jeffrey, Kate L and Reinecker, Hans-Christian}
}
