@article {1051161,
	title = {Mitochondrial Biogenesis and Proteome Remodeling Promote One-Carbon Metabolism for T Cell Activation},
	journal = {Cell Metab},
	volume = {24},
	number = {1},
	year = {2016},
	month = {2016 Jul 12},
	pages = {104-17},
	abstract = {Naive T~cell stimulation activates anabolic metabolism to fuel the transition from quiescence to growth and proliferation. Here we show that naive CD4(+) T~cell activation induces a unique program of mitochondrial biogenesis and remodeling. Using mass spectrometry, we quantified protein dynamics during T~cell activation. We identified substantial remodeling of the mitochondrial proteome over the first 24~hr of T~cell activation to generate mitochondria with a distinct metabolic signature, with one-carbon metabolism as the most induced pathway. Salvage pathways and mitochondrial one-carbon metabolism, fed by serine, contribute to purine and thymidine synthesis to enable T~cell proliferation and survival. Genetic inhibition of the mitochondrial serine catabolic enzyme SHMT2 impaired T~cell survival in culture and antigen-specific T~cell abundance in~vivo. Thus, during T~cell activation, mitochondrial proteome remodeling generates specialized mitochondria with enhanced one-carbon metabolism that is critical for T~cell activation and survival.},
	issn = {1932-7420},
	doi = {10.1016/j.cmet.2016.06.007},
	author = {Ron-Harel, Noga and Santos, Daniel and Ghergurovich, Jonathan M and Sage, Peter T and Reddy, Anita and Lovitch, Scott B and Dephoure, Noah and Satterstrom, F Kyle and Sheffer, Michal and Spinelli, Jessica B and Gygi, Steven and Rabinowitz, Joshua D and Sharpe, Arlene H and Haigis, Marcia C}
}
